Tick-Taq DNA Polymerase for PCR Applications and Downstream GMP Processes
Protean offers a genetically modified Tick-Taq DNA Polymerase that has been optimised for all routine PCR applications providing greater sensitivity and increased yields.
The Protean Tick-Taq DNA Polymerase comes with an optimized Taq 10x buffer containing 20 mM MgCl2.
Tick-Taq DNA Polymerase for Greater Sensitivity and High Yields
The Taq DNA Polymerase by Protean is a genetically modified variant of the original Taq DNA polymerase that is enhanced for polymerase chain reaction (PCR) applications. When compared to traditional Taq DNA polymerases, this Tick-Taq DNA Polymerase shows improved sensitivity, greater yields and provides longer PCR products.
With minimal refinement, Tick-Taq DNA Polymerase could be utilized with the same cycle settings as the regular Taq DNA polymerase.
Ultra-pure, High-processivity Recombinant Enzyme
Taq DNA polymerase is an ultra-pure, high-processivity recombinant enzyme. It has the His6 tag, which may be taken off easily for downstream applications. It is engineered with the patented Taq 10x buffer that contains KCl, (NH4)2SO4, and 20 mM MgCl2. 5 U/ul is the concentration.
A typical reaction configuration (25-45 cycles) is as follows: Initial Denaturation : 95°C 1-3min, Denaturation: 95°C 30 s, Annealing: Tm-5°C 30s, Extension*: 72°C 1 min, Final extension: 72°C 5–15 minutes * For PCR products up to 2 kb, a one-minute extension period is advised. The extension time should be increased by 1 minute per kilobyte for longer goods.
Tick-Taq DNA Polymerase Applications
Tick-Taq DNA Polymerase can be utilised for a variety of applications including PCR reactions, PCR labeling and effectively incorporating modified dUTP (Biotin, Digoxigenin, Cy5, Cy3), PCR Mutagenesis, Fill-in reactions, Primer extension and T/A cloning.
Taq DNA Polymerase for Downstream GMP Processes
All enzymes manufactured by Protean are ideal for use in pharma and biopharma manufacturing cGMP applications.
The Tick-Taq DNA Polymerase ultra-pure enzyme by Protean is produced in an ISO 9001 and ISO 13485 certified environment and is approved for use in downstream GMP certified manufacturing processes. The His6 tag can be removed easily for these purposes.
- Genetically modified Tick-Taq DNA Polymerase
- Optimised for all routine PCR applications
- Optimized Taq 10x buffer containing 20 mM MgCl2 included
- His6 tag can be removed for downstream processes
- Fully qualified for use in downstream GMP certified processes
For more information please contact Protean today or head to the online store and add to basket at Protean’s webstore: