Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT)

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Protean offers Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) enzyme isolated from Escherichia coli.

The M-MLV RT by Protean is ideal for most PCR-based amplification and detection methods.

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT)

The Moloney Murine Leukemia Virus encoded sequence of reverse transcriptase is optimized in silico and produced in E.coli strain. M-MLV RT is a highly active recombinant enzyme with increased thermal stability.

Most PCR-based amplification and detection methods require reverse transcription as a crucial first step in the analysis of RNA. The RNAse H-minus fragment of reverse transcriptase is suitable for all molecular techniques that need dependable reverse transcriptase activity, including RT-PCR, RT-qPCR and RT-LAMP assays.

Thermostable reverse transcriptase

The capacity of a reverse transcriptase to endure high temperatures is a key aspect of cDNA synthesis. Retroviral-derived reverse transcriptases are frequently not adequately thermostable to catalyze synthesis at temperatures high enough for the efficient conversion of RNAs that form stable secondary structures and double-stranded RNA targets.  

The high thermal stability of the Protean M-MLV RT enzyme enables coupled reverse transcription at elevated temperatures of up to 60°C (140°F). This results in increased sensitivity and specificity of various detection techniques. The concentration of the M-MLV RT is 200 U/ul.

In comparison with Avian Myeloblastosis Virus (AMV) Reverse Transcriptase, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) has low DNA endonuclease activity and RNase H activity.

M-MLV RT for cGMP manufacturing applications

All enzymes supplied by Protean, including M-MLV RT are produced in a certified environment under ISO 9001 and ISO 13485 standards and fully approved for use in cGMP certified manufacturing processes.

 RNAse H-minus


  • M-MLV RT isolated from E. coli
  • Thermostable reverse transcriptase active at up to 60°C (140°F).
  • Suitable for RT-PCR, RT-qPCR and RT-LAMP assays
  • Fully qualified for use in downstream GMP certified processes

For more information please contact Protean today or head to the online store and add to basket at Protean’s webstore:

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