Assay Biotechnology offers a comprehensive range of high-quality protein-specific ELISA Kits.
The enzyme-linked immunosorbent assay (ELISA) kits produced by Assay Biotechnology are highly reliable, sensitive, and ready-to-use.
High-Quality ELISA Kits
Assay Biotechnology has developed a diverse selection of high-quality ELISA Kits, including Cell-Based ELISA Kits, Sandwich ELISA Kits, Transcription Factor ELISA Kits, and others. Each kit undergoes meticulous development and rigorous quality control procedures to ensure precise and reproducible results, empowering researchers to confidently explore their respective fields of study.
The ELISA Kits are ready-to-use, providing all the necessary reagents for performing ELISA. This eliminates the need for fragmented procurement and ensures consistent, accurate, and reproducible results across various laboratory settings.
Cell-Based ELISA Kits
Assay Biotechnology offers a broad range of advanced Cell-Based ELISA Kits, which are reliable tools for researchers to gain valuable insights into intracellular processes. The Cell-Based ELISA Kits offer numerous advantages to researchers. Firstly, they provide a fast and cost-effective alternative to traditional Western blotting techniques. The kits offer direct and specific detection of the target protein of interest, ensuring no cross-reactivity with other proteins. Importantly, their sensitivity and reproducibility are high, with low background noise and a high signal-to-noise ratio. Moreover, the kits are highly flexible and adaptable, accommodating various cell types, treatments, and experimental conditions.
Colorimetric Cell-Based Phospho ELISA Kits enable researchers to measure the relative levels of phosphorylated proteins in cultured cells without lysing them. They are based on the principle of colorimetric detection using a 450 nm absorbance reader. These kits can help researchers to study the mechanisms and pathways of cellular signaling and response in various diseases and conditions.
The Colorimetric Non-Phospho Cell-Based ELISA Kits allow researchers to conveniently measure the relative levels of intracellular proteins in cultured cells without lysing them. Examples of non-phosphorylated proteins are transcription factors, which are involved in gene regulation and expression. Based on colorimetric detection using a 450 nm absorbance reader, the kits may be utilised to research transcriptional activation and repression in various diseases and conditions.
Fluorometric Phospho Cell-Based ELISA Kits allow researchers to measure the relative levels of phosphorylated proteins in cultured cells without lysing them. They are based on the principle of fluorometric detection using a fluorescence microplate reader. These kits can help researchers to understand the molecular mechanisms and pathways of diseases and conditions that involve protein phosphorylation.
Methyl, Acetyl and Cleaved Colorimetric Cell-Based ELISA Kits
Assay Biotechnology has also developed a range of other types of high-throughput lysate-free Colorimetric Cell-Based ELISA Kits, These include Colorimetric Cell-Based Methyl ELISA Kits for measuring the relative levels of methylated proteins, Colorimetric Cell-Based Cleaved ELISA Kits for measuring cleaved proteins, and Colorimetric Cell-Based Acetyl ELISA Kits for measuring the relative levels of acetylated proteins.
Sandwich ELISA Kits
Assay Biotechnology offers exceptional quality traditional Sandwich ELISA Kits, enabling accurate detection and quantification of target analytes.
These ELISA kits provide specific and sensitive detection of target proteins by utilising two antibodies that recognise different epitopes on the same protein. These kits offer quantitative and reproducible results and are designed to be easy and convenient to use, featuring pre-coated microstrips and a ready-to-use substrate and other necessary components.
Colorimetric Phospho Sandwich ELISA Kits utilise the quantitative technique of a “Sandwich” ELISA where the target protein (antigen) is bound in a “sandwich” format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The kits allow researchers to measure the relative levels of phosphorylated proteins in cell lysates or tissue extracts. They are based on the principle of colorimetric detection using a 450 nm absorbance reader.
Chemiluminescent Sandwich ELISA Kits allow researchers to measure the relative levels of proteins or protein complexes in cell lysates or tissue extracts. They are based on the principle of chemiluminescent detection using a luminol-based substrate and a horseradish peroxidase (HRP)-conjugated antibody or streptavidin. The kits can be more sensitive and stable than colorimetric detection methods, allowing for lower sample input and longer signal duration.
Transcription Factor ELISA Kits
Assay Biotech’s Transcription Factor ELISA Kits are designed for swift and effortless detection of active transcription factors in eukaryotic nuclear extracts or cell lysates. They are based on the principle of colorimetric detection using a 450 nm absorbance reader. The kits are specific and sensitive for the target transcription factor of interest. They are quantitative and reproducible and also offer the convenience of pre-coated plates and ready-to-use reagents.
Utilising the groundbreaking TFact™ DNA-Binding ELISA Kits can significantly reduce the runtime for detecting transcription factor binding to DNA elements, compared to EMSAs, chromatin immunoprecipitation, western blotting, and expression of fused target and reporter genes. The kits also eliminate the need for harmful radioactive labeling while maintaining high sensitivity and signal-to-noise ratio.
Colorimetric Non-Phospho Transcription Factor ELISA Kits are a type of ELISA Kits that allow researchers to measure the relative levels of non-phosphorylated transcription factors in eukaryotic nuclear or cell lysates. Example research applications for these kits include monitoring the expression and activation of COE2, which is a transcription factor that regulates neuronal differentiation and development, or ETV6, which is a transcription factor that is involved in hematopoiesis and leukemia.
Colorimetric Phospho Transcription Factor ELISA Kits enable researchers to measure the relative levels of phosphorylated transcription factors in eukaryotic nuclear or cell lysates. For example, they may be used to monitor the phosphorylation and activation of c-Fos, which is a transcription factor that forms part of the AP-1 complex and regulates cell proliferation and differentiation, or NF-κB p65, which is a transcription factor that regulates inflammatory and immune responses.
These products are for Research Purposes Only. Not Intended for Diagnostic or Clinical Procedures. For more information about the range of ELISA kits please contact Assay Biotechnology today or browse the full catalog: