High-Throughput Assay Kit for Albumin (HSA) Binding
The high-throughput Assay Kit for Albumin (HSA) Binding estimates the binding of drugs to human serum albumin and predicts the plasma protein binding in only 20 minutes.
Assay Kit for Albumin (HSA) Binding
The ready-to-go assay kit measures the affinity constant (KD) of
drugs to albumin and hence allows the calculation of albumin binding even under disease and physiological states that alter the albumin content of human serum. In combination with the TRANSIL AGP Binding Kit, it is possible to obtain a precise prediction of plasma protein binding in a highly controlled and reproducible assay environment.
Ready-to-go High-Throughput Assay Kit for Albumin (HSA) Binding
Sovicell has developed a dedicated ready-made assay kit for measuring the affinity of drug candidates to human serum albumin (HSA) to predict its free fraction in plasma – the TRANSIL HSA Binding Kit. This innovative assay kit for Albumin (HSA) binding offers a seamless workflow experience with its ready-made format and high-throughput capabilities.
Delivering results in just 20 minutes, the high-throughput HSA assay kit consists of ready-to-go 96-well microtiter plates. Each plate allows for the simultaneous assessment of HSA binding for up to 12 compounds, maximising efficiency and minimising resource consumption.
When used together with the TRANSIL AGP Binding Kit, the HSA Binding Assay Kit facilitates an accurate prediction of plasma protein binding in a highly controlled and reproducible assay environment.
Simulate a broad range of disease conditions with the HSA Binding and AGP Binding Assay Kits
Human serum albumin (HSA) and human a1-acid glycoprotein are the most important plasma binding proteins. TRANSIL binding assays are available for both proteins. The TRANSIL HSA Binding Kit employs immobilized HSA with a random orientation. This ensures that all binding sites are available and that the assay reproduces exactly the binding of drugs to albumin.
Furthermore, the high-throughput HSA Binding Assay Kit can be used as a rapid screen to predict plasma protein binding, and in conjunction with the TRANSIL AGP Binding assay also to measure plasma protein binding and to simulate various disease conditions. The differences in relation to plasma binding arise through variations in plasma composition, due to lipids blocking binding sites in native plasma, and occasionally due to binding to other plasma proteins with low abundance.
Estimating the binding of drugs to Albumin (HSA) with the five-step assay kit
The TRANSIL HSA Binding Assay kit estimates the binding of drugs in only 5 steps:
- addition of drug candidate
- mixing and incubating for 12 minutes
- removal of beads by centrifugation
- sampling of supernatant
- quantification of the drug candidate
Capabilities
Detection systems:
- LC/MS/MS
- Scintillation counting
- Others
Parameters estimated and predicted:
- Affinity constant (KD) of drugs to albumin
- Fraction bound to albumin
- Fraction to plasma
Features & Benefits of the High-Throughput Albumin (HSA) Binding Assay
- Fast, requires only 20 minutes total assay time
- Accurately measures the affinity of drug candidates to human serum albumin (HSA) to predict it’s free fraction in plasma
- Reliable with highly reproducible results and robust correlation to equilibrium dialysis method.
- Fully quality-controlled albumin binding estimates.
- Rapid compound quantification due to immobilized plasma proteins
- Includes a spreadsheet for calculation of final results and traffic light system for data quality rating
For more information please contact Sovicell today.
