Rapid Plasma Protein Binding Assay
The unique rapid plasma protein binding assay for sticky and tightly bound compounds can be used with plasma from any species.
The ready-to-use 96-well Plasma Protein Binding Assay revolutionises the assessment of plasma protein binding with its unparalleled efficiency. Unlike traditional methods, these assay kits utilise matrix-free technology, with binding proteins immobilized on beads, offering a multitude of advantages.
Plasma Protein Binding Assay
The Plasma Protein Binding Assay is a versatile and highly accurate, ready-to-use, high-sensitivity plasma protein binding kit that requires only 30 minutes of incubation time.
The TRANSIL High Sensitivity Binding Kit accurately determines the unbound fraction of drugs that are tightly bound to plasma proteins – even when the unbound fraction is well below 1%.
The rapid plasma protein binding assay has excellent recovery for drugs that exhibit too high unspecific binding in other assay systems or precipitate because of deficient solubility. It determines the fraction of the drug bound to plasma indirectly by measuring the partitioning of a drug between the plasma proteins and artificial cell membranes. With its unique capabilities, this assay can also be used for measuring plasma protein binding of a wide range of peptide drugs. This allows easy optimisation of peptide half-life by modulating their affinity to plasma.
Superior Method for Dose-finding
With unparalleled sensitivity and exceptional recovery rates, the TRANSIL High Sensitivity Binding Assay stands out as the preferred method for precisely determining the free fraction of a drug across all preclinical species. This capability is indispensable for effectively scaling dose-response and dose-toxicity relationships, crucial steps in preclinical research aimed at optimizing drug dosing strategies prior to initiating first-in-man studies.
Fast Downstream Analytical Processes
By eliminating the requirement for cumbersome separation procedures, the Plasma Protein Binding Assays significantly expedite downstream analytical processes. This streamlined workflow not only saves valuable time but also enhances efficiency in the laboratory. Researchers can bypass time-consuming steps, allowing for quicker data analysis and interpretation. Consequently, the accelerated analytical processes enable researchers to focus more on the interpretation of results and the progression of their research objectives, ultimately leading to faster insights and advancements in pharmaceutical research and development.
Advanced Precision and Efficiency in Protein Binding Studies
With rapid detection capabilities requiring a brief incubation time of no more than 12 minutes, researchers can efficiently explore protein binding interactions without the need for complex separation processes. The design ensures unbiased estimates, mitigating concerns related to ion suppression often encountered in LCMS/MS/MS analyses. Additionally, rigorous quality control measures, including accurate estimation of KD values for controlled plasma composition and maintenance of a stable pH environment to prevent measurement errors during dialysis, underscore the reliability of results. These user-friendly kits minimize manual labor, allowing researchers to easily add test items and incubate, with the added benefit of compatibility with automated systems for streamlined workflows. Sovicell’s Plasma Protein Binding Assay Kits represent a significant advancement in research capabilities, offering a dependable tool for enhancing understanding in preclinical research and drug discovery.
Six-step assay kit with pre-filled buffer and membrane beads
The assay kit consists of silanized glass vials pre-filled with buffer and membrane beads. It requires only 6 steps:
- addition of plasma
- addition of drug candidate
- incubation for 30 minutes while mixing
- removal of beads by centrifugation
- precipitation of plasma
- quantification of compounds
Capabilities
Detection systems:
- LC/MS/MS
- Scintillation counting
- Others
Parameters estimated:
- Fraction unbound in plasma (any species)
- Affinity to phosphatidylcholine membranes
Features & Benefits of the Plasma Protein Binding Assay Kits
- Fast, requires only 30 minutes of incubation time
- Accurate detection of very low free fractions (<1%)
- A versatile assay that can be used with plasma from any species
- High recovery even with very sticky drugs
- User-friendly assay system and easy handling
For more information please contact Sovicell today.
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