The Recombinant TEV Protease Protein His6 is a genetically optimised 28 kDa variation of the N1a protease from tobacco etch virus (TEV) that is designed to be more stable than native TEV protease with increased enzymatic activity.
Because of its high sequence specificity, the Tobacco Etch Virus (TEV) protease is a great tool for the cleavage of fusion proteins and removal of tags, such as maltose-binding protein (MBP) or poly-histidine, from recombinant proteins both in vitro and in vivo.
Genetically optimised 28 kDa version of the N1a protease from tobacco etch virus (TEV)
Proteolytic removal of the fusion carrier is a laborious process that can be expensive, tedious, and frequently results in products with additional amino acid residues to the native proteins. Using tobacco etch virus (TEV) protease with higher stringent sequence specificity will make the fusion protein approach considerably more feasible for protein drug research.
The Protean manufactured recombinant TEV Protease is highly specific and active for its seven-amino acid sequence with a minimal off-target impact. Its activity is more than 10,000 units per 1 mg protein. The activity depends on the type of target protein. The optimal amount of enzyme should be tested for each target protein.
TEV protease with increased sequence specificity and enzymatic activity
The genetically optimised 28 kDa recombinant TEV enzyme version of the N1a protease from tobacco etch virus (TEV) contains a His6 tag located at the N-terminus of the protein. This provides for an easy purification of the desired final protein by immobilization of the TEV Protease His6 on IMAC affinity resins and subsequent removal from the cleavage reaction.
The Recombinant TEV Protease Protein His6 is active over a wide range of pH values (4-8.5) and temperatures (4-34°C). The preferred recognition sequence is ENLYFQ|S(G,A), but it cleaves also motives EXLYFQ|S(G,A), where X could be any amino acid residue (in parenthesis are alternative residues). The Protean TEV substrate #1409 should be used as a positive control.
Cleavage of affinity tags from fusion proteins after protein purification
The Protean manufactured TEV Protease Protein His6, cleaves fusion proteins directly in a solution or immobilized on affinity resins. All enzymes supplied by Protean Ltd. are manufactured in the certified environment under stringent ISO 9001 and ISO 13485 international standards, which fully qualifies them for use in downstream GMP certified processes.
- Genetically optimised 28 kDa variation of the N1a protease
- High sequence specificity
- High enzymatic activity
- Active over a wide range of pH values (4-8.5)
- Active over a wide range of temperatures (4-34°C)
- Cleaves fusion proteins directly in a solution or immobilized on affinity resins
- Fully qualified for use in downstream GMP certified processes
- TEV Protease His6
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