T Cell Staining Reagents

imusyn’s Dyemer & myDyemer are a pioneering class of ready-to-use and loadable T Cell staining reagents, developed for precise quantification and enumeration of antigen-specific CD8+ T cells by flow cytometry.

A game-changer in T cell research, the innovative T cell staining reagents are generated from recombinant Major Histocompatibility Complex (MHC) class I proteins that undergo expression in mammalian cell culture to guarantee the integrity of protein glycosylation and accurate binding and recognition.

T Cell Staining Reagents

T cells play a key part in immune surveillance, coordinating responses to infections, cancer, and autoimmune diseases. imusyn’s groundbreaking Dyemer and myDyemer T Cell staining reagents enable exceptionally fast and precise analysis of T cell dynamics using flow cytometry.

Advanced T Cell Staining with Dyemer

Dyemer and myDyemer signify a remarkable advancement in T Cell staining. Engineered with recombinant MHC class I proteins that undergo expression in mammalian cell culture, the integrity of protein glycosylation is ensured for biological relevance.

The innovative Dyemer T cell staining reagent enables the efficient detection and quantification of antigen-specific CD8+ T cells by flow cytometry. By multimerising peptide-MHC (pMHC) complexes onto fluorophore-labeled streptavidin, it facilitates precise surface staining of T cells. The identification of these complexes by specific T cell receptors enables the quantification of antigen-specific CD8+ T cells in a variety of biological samples. Another remarkable feature of imusyn’s staining reagents is the straightforward removal of the staining complex from the cell surface by simple biotin incubation. imusyn’s T cell staining reagents undergo rigorous quality control to ensure consistent performance across batches. 

In addition to the Standard Panel Dyemers, researchers can request custom-made Dyemers, precisely loaded with your target peptides confirmed by imusyn’s Class I Peptide Binding Analysis. 

One-step Peptide Loading with myDyemer

myDyemer provides unprecedented flexibility by allowing researchers to load specific peptides onto pre-multimerised and peptide-receptive MHC class I molecules. Unlike traditional methods, myDyemers remain stable even in the absence of peptides.

With a simple one-step peptide addition, researchers can effortlessly load their peptide of interest, eliminating the need for laborious and time-consuming refolding assays or peptide exchanges. This technique offers remarkable convenience and enables a swift experimental setup, whilst minimising the probability of errors during manual preparation.

With myDyemer, researchers can gain access to a vast array of T cell specificities, making detection and screening significantly easier and more efficient. Plus, with the extensive range of MHC class I alleles available at imusyn, researchers’ unique needs are met with advanced precision.

Revolutionising Detection of Antigen-specific T Cells by Flow Cytometry

imusyn’s Dyemer and myDyemer set the standard for detecting antigen-specific CD8+ T cells with unparalleled reliability and efficiency. In comparative studies against other T cell staining reagents like tetramers (Figure 1), imusyn’s Dyemer consistently delivers exceptional results.

T Cell  Staining Efficiency
Figure 1: Staining efficiency of imusyn’s Dyemer compared to a tetramer of a competitor. Staining has been performed on healthy donor PBMCs gated for CD8+ T cells and incubated with PE-labeled Dyemer or tetramer of the same specificity.

T Cell Staining Reagents for Cutting-Edge Research

imusyn’s T cell staining reagents can accelerate research across numerous scientific domains, including transplantation, autoimmunity, immuno-oncology and immunology. They perform a pivotal role in cellular therapies as their precision and efficiency make them indispensable for flow cytometry applications.

Dyemer and myDyemer enable the precise measurement of T cell responses and immune monitoring, helping researchers understand host-pathogen interactions, vaccine efficacy and disease mechanisms. Employing the reagents to analyse T cell responses in the tumor microenvironment, researchers can gain advanced insights into the interplay between cancer cells and the immune system, accelerating the development of novel immunotherapies and predictive biomarkers.

Peptide Binding Verification with imusyn’s imuPep

Researchers can gain complete assurance regarding peptide binding to specific HLA class I alleles to validate their binding predictions.

Leveraging imusyn’s long-standing expertise, researchers can benefit from the company’s Class I Peptide Binding Analysis – imuPep. By loading recombinant HLA class I molecules with specific peptides, imusyn can validate peptide binding through meticulous monitoring of thermal stability of the peptide/HLA class I complex.

T Cell Staining Reagents

Features of the T Cell Staining Reagents:

  • Off-the-shelf or custom-made Dyemers
  • Peptide-receptive myDyemers for flexible self-loading
  • Derived from recombinant HLA and H2 class I proteins
  • Expressed in mammalian cell culture
  • Reversible staining
  • Targeted quantification of CD8+ T cells
  • Vast diversity of available alleles

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