Stat6 (Phospho-Tyr641) Antibody

The Stat6 (Phospho-Tyr641) Antibody from Assay Biotechnology is a polyclonal antibody that specifically recognizes the phosphorylated form of Stat6 at Tyr641. It has been extensively validated for Western blotting (WB), immunohistochemistry on paraffin-embedded sections (IHC-p), immunofluorescence for paraffin-embedded tissue sections (IF paraffin section), and enzyme-linked immunosorbent assay (ELISA) applications.

This Phospho Specific antibody can detect Stat6 (Phospho-Tyr641) in human, mouse, and rat tissues and cells. 

Stat6 (Phospho-Tyr641) Antibody

The Stat6 (Phospho-Tyr641) Antibody from Assay Biotechnology is a polyclonal antibody that specifically recognizes the phosphorylated form of Stat6 at Tyr641. Stat6 is a member of the STAT family of transcription factors and plays a vital role in mediating cellular responses to cytokines and growth factors. Upon activation by interleukin-4 (IL-4) and other signaling molecules, Stat6 undergoes phosphorylation by receptor-associated kinases and forms homo- or heterodimers. These dimers then translocate to the nucleus, where they act as transcription activators, influencing the expression of genes involved in IL-4-mediated biological responses.

Importance and Function of Stat6

Stat6 is known to induce the expression of BCL2L1/BCL-X(L), a key protein responsible for the anti-apoptotic activity of IL-4. Knockout studies in mice have demonstrated its involvement in T helper 2 (Th2) cell differentiation, cell surface marker expression, and class switch of immunoglobulins. Dysregulation of Stat6 signaling has been implicated in various diseases, highlighting the significance of understanding its phosphorylation status and activity.

Reactivity in Human, Mouse and Rat Samples

The Stat6 (Phospho-Tyr641) Antibody demonstrates broad reactivity, making it suitable for use in various species including human, mouse, and rat. This broad reactivity allows researchers to investigate Stat6 phosphorylation and activity across different model organisms, facilitating translational research and comparative studies. By utilizing this antibody, researchers can explore the similarities and differences in Stat6 signaling among different species, providing comprehensive insights into its function and regulation. The antibody’s reactivity in multiple species enhances its versatility and applicability in diverse research areas, including immunology, cancer biology, and developmental biology, among others.

High Specificity – Detects endogenous levels of Stat6 protein only when phosphorylated at Y641

The Stat6 (Phospho-Tyr641) Antibody exhibits exceptional specificity in detecting the phosphorylated form of Stat6 at Tyr641. It recognizes endogenous levels of Stat6 protein only when phosphorylated at this specific tyrosine residue. This high specificity ensures accurate and reliable detection of phosphorylated Stat6 and minimizes the potential for cross-reactivity with non-phosphorylated Stat6 or other proteins. By selectively targeting the phosphorylated form of Stat6, researchers can confidently assess its activation status and gain insights into the phosphorylation-dependent signaling events mediated by Stat6. This specificity is crucial for obtaining precise and meaningful data, enabling a better understanding of Stat6-mediated cellular responses and their role in various physiological and pathological processes.

Purity and Concentration

The Stat6 (Phospho-Tyr641) Antibody from Assay Biotechnology has undergone affinity purification from rabbit antiserum using epitope-specific immunogen and affinity chromatography. This purification method ensures enhanced specificity and reduces nonspecific binding of the Stat6 (Phospho-Tyr641) Antibody, resulting in more accurate and reliable results.

The antibody has a concentration of 1 mg/ml and a molecular weight of approximately 94,135 Da. It is supplied in a liquid formulation containing phosphate-buffered saline (PBS), 50% glycerol, 0.5% bovine serum albumin (BSA), and 0.02% sodium azide as preservatives. This formulation enhances the stability and longevity of the antibody, allowing for long-term storage at -20°C for up to one year.

Storage and Stability

The formulation of the antibody is carefully optimized to maximize its stability and longevity, allowing for long-term storage and repeated usage. It is advised to store the antibody at -20°C, and it maintains its quality for up to one year when stored under appropriate conditions. The antibody is provided with a detailed Product Datasheet and Safety Datasheet that provide further information regarding product specifications, experimental protocols, and quality control.

Performance and Reliability in Experimental Settings

Application data demonstrates the high sensitivity and specificity of the Stat6 (Phospho-Tyr641) Antibody in different experiments and sample types. For instance, it can detect endogenous levels of Stat6 (Phospho-Tyr641) in IL-4-treated HeLa cells by WB, exhibit strong staining of Stat6 (Phospho-Tyr641) in human breast carcinoma by IHC, and measure the amount of Stat6 (Phospho-Tyr641) in immunogen phosphopeptide by ELISA.

The Stat6 (Phospho-Tyr641) Antibody from Assay Biotechnology is a valuable resource for researchers interested in studying Stat6 signaling and function across various biological systems. By utilizing this antibody, researchers can gain valuable insights into the expression, localization, regulation, and activity of Stat6 in both normal and pathological conditions. Its versatility, reliability, and high-quality results make it an ideal choice for investigating the role of Stat6 in cytokine-mediated responses and various diseases.

Specifications Summary – Stat6 (Phospho-Tyr641) Antibody

Assay Biotechnology – Catalog No: A7229

  • Reactivity: Human, Mouse, Rat
  • Applications: WB, IHC-p, IF(paraffin section), ELISA
  • Modification: Phospho Specific
  • Source: Polyclonal Rabbit
  • Storage and Stability: -20°C/1 year

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