Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) Services
ZentriForce Pharma offers specialised Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) services – widely recognised as the gold standard for aggregation and heterogeneity analysis.
As a first-principle method, SV-AUC delivers highly reliable data in a single run, including:
- Relative concentrations of sample components
- Sedimentation coefficients
- Molecular weights
- Shape information (e.g., frictional ratio)
- Interaction parameters (B22, ks)
SV-AUC: The Gold Standard in Biopharmaceutical Characterisation
Because samples are analysed directly in solution, SV-AUC preserves the native composition and distribution of species. Regulatory agencies such as the FDA and EMA increasingly emphasise SV-AUC data in submissions, making it an essential tool for biopharmaceutical development and biosimilarity studies.
Why Choose SV-AUC Services at ZentriForce Pharma?
- Extensive expertise: A specialised scientific team with deep hands-on experience across proteins, peptides, nucleic acids, nanoparticles, viruses, and gene therapy products.
- Flexible sample handling: BSL-1 and BSL-2 environments, supporting a wide range of biopharmaceutical materials.
- Efficient throughput: Up to 14 samples per run with optimised workflows to reduce costs per sample.
- Advanced analysis: Data evaluation with Ultrascan, processed on our in-house computer cluster for high-resolution, secure, and reliable results.
- Quality assured: Robust SOPs, trained staff, regularly maintained equipment, and full audit support ensure regulatory compliance and data integrity.
Technical Specifications
| Parameter | Specification |
| Instrument | Optima Analytical Ultracentrifuge – high resolution and reproducibility |
| Outputs | Sedimentation coefficient distribution (s), molecular weight (Mw), relative concentrations, frictional ratio (f/f₀), interaction parameters (B22, ks) |
| Size range | 0.5 nm – 200 nm; 0.5 kDa up to upper limits defined by particle size |
| Sample requirements | 400 µL per run, measured in 12 mm AUC cells with Sapphire windows |
| Temperature control | 4 °C to 40 °C for versatile applications |
Applications of SV-AUC
Biosimilarity Studies
ZentriForce Pharma routinely applies SV-AUC in biosimilarity assessments, for example, for the comparison of monoclonal antibodies and biosimilar candidates. The method reliably quantifies monomer vs. aggregate content, detects fragments, and confirms active pharmaceutical ingredient (API) integrity. Regulators such as the FDA and EMA increasingly expect SV-AUC data as part of biosimilarity submissions.
Biopharmaceutical Development
In early- and late-stage development, SV-AUC supports formulation screening and stability testing by measuring aggregation tendencies, self-association (B22, ks), and molecular weight distributions. Our team has applied SV-AUC across a wide range of therapeutic proteins, enzymes, and nanoparticles, delivering high-resolution data to guide development decisions.
Gene Therapy Development
SV-AUC uniquely separates species by size and density simultaneously, enabling precise measurement of full vs. empty viral particles and other critical quality attributes in gene therapy vectors such as AAV, viruses, and nucleic acid complexes.
Peptide & Oligonucleotide Analysis
Peptides and oligonucleotides are analytically challenging due to their intermediate size. SV-AUC provides clear resolution of purity and composition, with advanced analysis enabling detection of impurities and aggregates as small as 1 kDa.
Partner with ZentriForce Pharma
By integrating SV-AUC with complementary techniques such as SEC-MALS, AF4, μ-DSC, and others, ZentriForce Pharma delivers a complete portfolio of analytical solutions for biopharmaceutical development.
ZentriForce Pharma’s expertise, advanced infrastructure, and regulatory-ready quality system make them a trusted partner for companies seeking accurate, cost-efficient, and high-resolution SV-AUC data.
📩 Contact ZentriForce Pharma today to discuss how SV-AUC can support your development program. Please email ZentriForce at info@zentriforce.com or complete the Quick Contact Form
