The Patched Antibody from Assay Biotechnology is a polyclonal antibody that recognizes the Patched protein, a receptor for the hedgehog family of secreted proteins. It has been extensively validated for various applications, including Western blotting (WB), immunohistochemistry on paraffin-embedded sections (IHC-p), immunofluorescence for paraffin-embedded tissue sections (IF paraffin section, and enzyme-linked immunosorbent assay (ELISA).
The Patched Antibody can be used for detecting the expression and localization of Patched in both human and mouse biological samples.
The Patched Antibody from Assay Biotechnology is a reliable and versatile tool for detecting and quantifying the expression and localization of Patched in different biological samples and applications. It is a high-quality polyclonal rabbit antibody that specifically recognizes the Patched protein, also known as protein patched homolog 1 (PTCH1), which is a key component of the hedgehog signaling pathway.
Patched interacts with the hedgehog ligands, such as sonic hedgehog, desert hedgehog, and Indian hedgehog, and modulates the activity of the downstream signaling molecule Smoothened. Mutations or dysregulation of Patched have been implicated in various cancers and developmental disorders, such as basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, and holoprosencephaly. Therefore, studying the expression and function of Patched is important for understanding the molecular mechanisms and pathological consequences of hedgehog signaling. The Patched Antibody can be used for detecting the expression and localization of Patched in different biological samples and applications. It has been validated for WB, IHC-p, IF (paraffin section) and ELISA applications.
Reactivity in Human and Mouse Samples
The Patched Antibody from Assay Biotechnology can detect Patched in human and mouse tissues and cells with high sensitivity and specificity. This enables researchers to achieve accurate and reliable results in different experiments and applications.
High Specificity – Detects endogenous levels of Patched protein
The Patched Polyclonal Antibody has the ability to recognize and bind to the Patched protein that is endogenously expressed by the cells, and not affected by external factors or modifications. This specificity allows the researchers to study the Patched protein that is biologically active and relevant for the hedgehog signaling pathway in different tissues, cells, and conditions. It can help the researchers to gain insights into the expression, localization, regulation, and function of Patched in normal and pathological processes.
Purity and Concentration
The antiserum contains polyclonal antibodies that were generated by immunizing rabbits with a synthetic peptide that matches the N-terminal region (amino acids 1-50) of human Patched, which enhances the specificity and affinity of the antibodies for the Patched protein. The antibody has a concentration of 1 mg/ml. It is supplied in a liquid form in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage and Stability
The formulation is enhanced for optimal stability and longevity of the antibody, making it suitable for long-term storage and repeated use. It is recommended to store the antibody at -20°C, and it has a shelf life of up to one year under appropriate storage conditions. The antibody is provided with a detailed Product Datasheet and Safety Datasheet that provide further information regarding product specifications, experimental protocols, and quality control.
Performance and Reliability in Experimental Settings
Application data showcases the results of the antibody in different experiments and sample types, demonstrating its high sensitivity and specificity. For example, the antibody can detect endogenous levels of Patched in mouse muscle cells by WB, show strong staining of Patched in human brain tissue by IHC, reveal the subcellular localization of Patched in human kidney tissue by IF, and measure the amount of Patched in human serum by ELISA.
The Patched Antibody from Assay Biotechnology is a useful resource for researchers who are interested in studying hedgehog signaling and function in various biological systems. By using this antibody, researchers can gain insights into the expression patterns, subcellular distribution, interactions, regulation, and activity of Patched in normal and pathological conditions. The antibody can also be used for screening potential modulators or inhibitors of Patched or hedgehog signaling for therapeutic purposes. The antibody is easy to use, reliable, and versatile, and can provide high-quality results in different assays and sample types.
Specifications Summary – Patched Antibody
Assay Biotechnology – Catalog No: C0296
- Reactivity: Human, Mouse
- Applications: WB,IHC-p, IF(paraffin section),ELISA
- Modification: Unmodified/Total
- Source: Polyclonal Rabbit
- Storage and Stability: -20°C/1 year
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