TGF-beta3 (Human) OmniKine™ ELISA Kit

The TGF-beta3 (Human) OmniKine™ ELISA Kit is a comprehensive solution designed to accurately measure the levels of natural or recombinant TGF-beta3 in various experimental samples, including cell lysates, serum, and plasma. This kit provides all the essential components required for quantitative determination, ensuring precise and reliable results for TGF-beta3 concentration analysis.

Human TGF-beta3
Colorimetric Sandwich ELISA Kit

TGF-beta3 is a member of the TGF-beta family of proteins that play crucial roles in embryogenesis, cell differentiation, wound healing, tissue repair, and fibrosis. Mutations in TGF-beta3 can lead to familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1), a cardiac disorder characterized by ventricular arrhythmias and sudden death.

The TGF-beta3 (Human) OmniKine™ ELISA Kit offered by Assay Biotechnology is a reliable and sensitive sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of human transforming growth factor beta-3 (TGF-beta3) in supernatants, sera, and plasma samples. This kit provides researchers with a powerful tool to study the expression, function, and regulation of human TGF-beta3 in various biological systems and conditions.

The TGF-beta3 OmniKine™ ELISA Kit is compatible with various sample types, including plasma, serum, and cell culture supernatant. This versatility allows researchers to analyze TGF-beta3 levels in a wide range of experimental samples relevant to their specific research interests.

Excellent sensitivity and dynamic range for measuring TGF-beta3 concentrations

With a wide detection range of 32-2000 pg/ml, this ELISA kit offers excellent sensitivity and dynamic range for measuring TGF-beta3 concentrations. The assay can be completed in just 4.5 hours, allowing for efficient and timely experimental workflows. Additionally, the kit has been extensively validated for linearity, recovery, precision, and specificity using various biological samples, ensuring accurate and reproducible results.

Detects both recombinant and naturally produced Human TGF-beta3 proteins

The OmniKine™ Human TGF-beta3 ELISA is a highly versatile assay that effectively detects both recombinant and naturally produced Human TGF-beta3 proteins. Rigorous testing has been conducted on various antigens at a concentration of 50 ng/ml to assess cross-reactivity and interference. The results demonstrated that the assay showed negligible cross reactivity or interference with the following antigens: Latent TGF-beta1, TGF-beta1, TGF-beta1.2, TGF-beta2, and TGFbeta R2. This ensures the reliability and specificity of the assay in accurately measuring Human TGF-beta3 levels.

Ready-to-use: The TGF-beta3 OmniKine™ ELISA Kit includes all the necessary components

The TGF-beta3 OmniKine™ ELISA Kit includes all the necessary components to perform the assay. It consists of pre-coated microplates, standards, detection antibodies, enzyme conjugates, substrate solution, stop solution, and wash buffer. The assay is based on the principle of capturing human TGF-beta3 using specific antibodies coated on the microplate wells and detecting it with biotinylated antibodies and streptavidin-horseradish peroxidase (SA-HRP) conjugate. The resulting colorimetric signal is proportional to the amount of human TGF-beta3 present in the sample and can be measured at 450 nm using a spectrophotometer.

The TGF-beta3 OmniKine™ ELISA Kit also comes with a detailed protocol and safety datasheet. These documents provide essential information about the product specifications, quality control procedures, and performance characteristics. This ensures that you have all the necessary information to successfully perform the assay and interpret the results.

The TGF-beta3 (Human) OmniKine™ ELISA Kit by Assay Biotechnology is a valuable tool for researchers studying the expression, function, and regulation of human TGF-beta3. With its ease of use, reliability, sensitivity, and wide range of applications, this ELISA kit enables accurate and high-quality measurements of TGF-beta3 concentrations in various biological samples.

Specifications Summary – TGF-beta3 (Human) OmniKine™ ELISA Kit

Assay Biotechnology – Catalog No: OK-0265

• Reactivity: Human
• Assay Type: Sandwich ELISA
• Format: 1 plate: 12 x 8-Well Microstrips
• Dynamic Range: 32-2000 pg/ml

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