Nanoparticle Analysis Services

ZentriForce Pharma provides an integrated suite of nanoparticle analysis services, offering advanced characterisation for biotherapeutics, gene therapy vectors, and lipid-based formulations.

Comprehensive Nanoparticle Analysis Services

The analytical platform encompasses Nanoparticle Tracking Analysis (NTA), Asymmetrical Flow Field-Flow Fractionation (AF4), Analytical Ultracentrifugation (AUC), Dynamic Light Scattering (DLS), and Size Exclusion Chromatography coupled with Multi-Angle Light Scattering (SEC-MALS).

Together, these complementary techniques enable detailed assessment of particle size distribution, concentration, and aggregation, ensuring reliable data for research and regulatory applications.

Key Nanoparticle Analysis Techniques

Nanoparticle Tracking Analysis (NTA)

NTA combines light scattering and Brownian motion to determine particle size and concentration in real time with single-particle resolution.
Measurements are performed using the NanoSight NS300 (Malvern Panalytical), offering temperature control and visual confirmation of particle behaviour.

The NanoSight NS300 provides high-resolution size and concentration analysis of nanoparticles, quantitative and qualitative insights for heterogeneous samples, temperature-controlled measurements for samples in diameter range: 20 nm – 1000 nm.

Asymmetrical Flow Field-Flow Fractionation (AF4)

Separates nanoparticles in the 1 nm–1 µm range by hydrodynamic diameter, providing particle size distribution, molecular weight, and aggregation data when combined with detectors such as MALS and RI. Ideal for complex biological formulations, including liposomes, LNPs, and nanoscale delivery vehicles.

Analytical Ultracentrifugation (AUC)

Delivers high-resolution insights into molecular size, stability, and aggregate formation through sedimentation velocity analysis, supporting the evaluation of viral vectors, LNPs, and nanoparticles in their native state.

Dynamic Light Scattering (DLS)

Measures hydrodynamic particle size and polydispersity in solution, facilitating rapid screening of formulations and stability studies for biotherapeutics and gene delivery systems.

Size Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS)

Determines absolute molecular weight, oligomeric state, and aggregation profile of nanoparticle formulations, without reliance on calibration standards.

Videodrop analysis

Videodrop combines particle tracking analysis and Interferometric video microscopy. It is the ideal tool for lentiviral vector (LV) development, extracellular vesicle (EVs) research and production, lipid nanoparticle (LNP) formulation. Also Videodrop is able to measure a temporal evolution of size like nanoparticle aggregation or complexation for Transfection Mix (TM).

Key Applications and Focus Areas

Biotherapeutics
Characterisation of protein- and lipid-based therapeutics for molecular size, purity, and aggregation, ensuring formulation quality and structural consistency.

Gene Therapy
Analysis of adeno-associated viruses (AAVs) and other viral vectors for size, integrity, and purity, enabling informed decision-making during development and production.

Biosimilarity Studies
Generation of comparative analytical data to demonstrate structural and purity equivalence between biosimilar and reference products.

Aggregate Detection
Quantitative assessment of soluble aggregates — a critical quality attribute influencing product stability and immunogenicity.

Drug Delivery Systems
Comprehensive characterisation of lipid nanoparticles (LNPs), liposomes, and other nanoscale delivery vehicles for drug encapsulation and release studies.

Partner with ZentriForce Pharma

By integrating techniques such as NTA, AF4, SEC-MALS, SV-AUC, DLS, Videodrop and µ-DSC within a unified orthogonal framework, ZentriForce Pharma delivers comprehensive nanoparticle characterisation for research, development, and regulatory submission.

All analyses are conducted under a WHO-aligned quality management system, governed by nearly 30 standard operating procedures (SOPs) covering methods, equipment, and data integrity. Work is performed in BSL-1 and BSL-2 laboratories, ensuring safe handling of a wide range of biological materials, with full audit-readiness and data traceability.

📩 Contact ZentriForce Pharma Research today to learn how its nanoparticle analysis capabilities can support drug development and quality assurance objectives. Please email info@zentriforce.com or complete the Quick Contact Form