Cellendes offers a range of fast gelling hydrogel kits for 3D Cell Culture Environments.
The 3-D Life Fast Gelling (FG) Kits provide a complete set of reagents for creating cell-compatible, fast gelling hydrogels for the creation of extracellular microenvironments for 3D cell culture.
Fast Gelling Hydrogels for Rapid Gelation
The 3-D Life Hydrogel System facilitates a broad variety of cell culture applications as it offers total control over biomolecular modifications and gel stiffness.
The polymers Mal-Dextran and Mal-PVA are utilised along with crosslinkers (PEG-Link and CD-Link) to create hydrogels that gel rapidly. The incubation time needed for the attachment of adhesion peptides is only 5 minutes and the gel’s formation happens right away, within seconds or a few minutes based on the gel composition and the applied pH.
The CD-Link crosslinker is made of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala). The MMP-cleavable peptide is developed for a variety of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9. It enables cells to spread and migrate within the hydrogel if they express the particular MMPs. Commonly, the spreading and migration of cells also require the presence of adhesion peptides.
Fast gelling hydrogels can be used in an automated setup of cultures in multiwell plates and have been shown to be suited for drug screening (Rimann et al., 2014).
The Benefits of Fast Gelling Gels
Fast gelling hydrogels are used when the formation of hydrogels needs to happen very quickly. For instance, when cells need to obtain an appropriate medium within a few minutes, or when the application requires very rapid gelation, such as for specific cases of 3D bioprinting.
Fast gelation prevents sedimentation of cells or tissue pieces and keeps them well distributed throughout the gel. To provide sufficient time for manually placing the gel, the formation should be performed at pH 5.5. For immediate gel formation, CB pH 7.2 should be used.
The 3-D Life Fast Gelation (FG) Hydrogel Range
The 3-D Life fast gelation (FG) hydrogel range includes:
3-D Life Dextran-PEG Hydrogel FG
3-D Life Dextran-CD Hydrogel FG
3-D Life PVA-PEG Hydrogel FG
3-D Life PVA-CD Hydrogel FG
Optional: 3-D Life RGD Peptide (Catalog Number 09-P-001)
The Dextran-based hydrogels can be dissolved by adding 3-D Life Dextranase (Cat. No. D10-1), allowing the recovery of chemically fixed or live cells for post-culture analyses, such as RT-PCR, or for further cultivation.
- Fast gelling (FG) hydrogels
- Complete set of reagents
- Complete control of biomolecular modifications
- 3D cultivation of different cell types, spheroids, tissue samples
- Non-toxic crosslinking chemistry
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