IRAK-1 (Phospho-Thr209) Antibody
The IRAK-1 (Phospho-Thr209) Antibody from Assay Biotechnology is a highly specific polyclonal antibody designed to target the interleukin-1 receptor-associated kinase 1 (IRAK-1).
The antibody exhibits exceptional specificity and detects endogenous levels of IRAK-1 protein only when phosphorylated at T209.
IRAK-1 (Phospho-Thr209) Antibody
The Assay Biotechnology IRAK-1 (Phospho-Thr209) Antibody is a polyclonal antibody with high specificity for the interleukin-1 receptor-associated kinase 1 (IRAK-1), a protein involved in inflammatory signaling. IRAK-1 is one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene encodes IRAK-1, which plays a critical role in IL1-induced upregulation of the transcription factor NF-kappa B. IRAK-1 acts as a key mediator of downstream signaling cascades, contributing to the modulation of immune responses and inflammatory processes. The IRAK-1 (Phospho-Thr209) Antibody allows researchers to investigate the function and regulation of IRAK-1 in cellular signaling pathways, immune responses, and disease mechanisms.
Reactivity in Human, Mouse, and Rat Samples
The IRAK-1 (Phospho-Thr209) Antibody is designed to detect and localize IRAK-1 protein in human, rat, and mouse tissues and cells. This broad reactivity enables researchers to study IRAK-1 in diverse biological systems and model organisms, facilitating translational research and comparative studies. The antibody has been extensively validated for Western blotting (WB) and enzyme-linked immunosorbent assay (ELISA) applications.
High Specificity – Detects endogenous levels of IRAK-1 protein only when phosphorylated at T209
The Assay Biotechnology IRAK-1 (Phospho-Thr209) Antibody has undergone extensive validation to ensure high specificity in detecting IRAK-1 protein specifically phosphorylated at T209, a critical residue for its inflammatory signaling function.
The antiserum used to generate the IRAK-1 (Phospho-Thr209) Antibody is produced against a synthesized peptide derived from human IRAK1 around the phosphorylation site of Thr209. The peptide spans the amino acid range of 175-224, ensuring specific recognition of the phosphorylated form of IRAK-1 at Thr209. This production method enhances the antibody’s specificity and sensitivity, allowing for accurate detection and analysis of phosphorylated IRAK-1.
T209 phosphorylation initiates the activation of IRAK-1, which then gets further phosphorylated and released from the receptor complex to interact with downstream molecules. This antibody allows one to track the activation of IRAK-1 under different stimuli and conditions, and investigate its role in inflammation, cancer progression, and therapy resistance. This antibody is more sensitive and specific than antibodies that target unmodified or total IRAK-1 protein, which may not indicate its functional state.
Purity and Concentration
The IRAK-1 (Phospho-Thr209) Antibody is affinity-purified from rabbit antiserum using epitope-specific immunogen derived from the phosphorylation site of Thr209 on human IRAK-1 protein. This purification process ensures enhanced specificity and minimizes nonspecific binding, providing reliable and accurate results. The antibody has a concentration of 1 mg/ml and is supplied in a liquid formulation containing 50% glycerol, 0.5% bovine serum albumin (BSA), and 0.02% sodium azide. This formulation enhances the stability and longevity of the antibody, making it suitable for long-term storage and repeated use.
Storage and Stability
The IRAK-1 (Phospho-Thr209) Antibody is supplied in a liquid formulation containing phosphate-buffered saline (PBS), 50% glycerol, 0.5% bovine serum albumin (BSA), and 0.02% sodium azide. This formulation serves multiple purposes to ensure the stability and longevity of the antibody. The PBS buffer helps maintain the antibody’s integrity, while the addition of glycerol acts as a cryoprotectant, safeguarding the antibody during freezing and thawing cycles. The presence of BSA reduces non-specific binding, minimizing background noise and enhancing the antibody’s specificity. The inclusion of sodium azide prevents bacterial growth, preserving the antibody’s quality over time. Together, these components create an optimized liquid formulation that enhances the stability, convenience, and reliability of the IRAK-1 (Phospho-Thr209) Antibody for long-term storage and repeated experimental use.
For long-term stability, it is recommended to store the antibody at -20°C, and it has a shelf life of up to one year under appropriate storage conditions. The antibody is provided with a comprehensive Product Datasheet and Safety Datasheet that provide detailed information regarding product specifications, experimental protocols, and quality control.
Performance and Reliability in Experimental Settings
The performance and reliability of the IRAK-1 (Phospho-Thr209) Antibody have been demonstrated through extensive application data. These data showcase the high sensitivity and specificity of the antibody in various experiments and sample types. Some examples include Western blot analysis of cell lysates and ELISA for quantitative detection of phosphorylated IRAK-1 protein. These results highlight the antibody’s robust performance and its suitability for studying IRAK-1 in different experimental contexts.
The IRAK-1 (Phospho-Thr209) Antibody from Assay Biotechnology offers researchers a highly specific tool to investigate the role of IRAK-1 in inflammation, cancer progression and therapy resistance. This antibody can help researchers to understand the molecular mechanisms of IRAK-1 in cancer biology and to identify potential therapeutic targets for cancer treatment.
Specifications Summary – IRAK-1 (Phospho-Thr209) Antibody
Assay Biotechnology – Catalog No: A1074
- Reactivity: Human, Rat, Mouse
- Applications: WB, ELISA
- Modification: Phospho Specific
- Source: Polyclonal Rabbit
- Storage and Stability: -20°C/1 year
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