Selective Media in Plates for Microbiological Testing

PharmaMedia Dr. Müller (PMM) offers a range of selective media in plates for microbiological testing in the pharmaceutical industry. The portfolio is designed for the examination of non-sterile products and testing for specified microorganisms, with individual media available for applications including the detection of Pseudomonas aeruginosa, Burkholderia cepacia complex, Staphylococcus aureus, Escherichia coli, bile-tolerant Gram-negative bacteria, and Salmonella spp.

The range also includes R2A Agar for the detection of microorganisms from water for injections in bulk, highly purified water, and purified water in bulk, as well as the detection of aerobic heterotrophic microorganisms from low-nutrient environments.

PMM selective media are single-bagged and non-irradiated and are supplied ready to use in 90 mm plates. Every batch can be obtained with a Certificate of Analysis (CoA).

Selective Culture Media for Pharmaceutical Microbiology

Selective culture media are used to support the examination of non-sterile products and the testing of specified microorganisms. PMM provides a portfolio of ready-to-use selective agar plates covering a range of microbiological testing applications relevant to pharmaceutical laboratories.

The portfolio includes:

• Cetrimide Agar for testing for Pseudomonas aeruginosa
• Burkholderia cepacia Complex Selective Agar (BCSSA) for testing for Burkholderia cepacia complex
• Mannitol Salt Agar for testing for Staphylococcus aureus
• MacConkey Agar for testing for Escherichia coli
• VRBD Agar for testing for bile-tolerant Gram-negative bacteria
• XLD Agar for testing for Salmonella spp.
• R2A Agar for detection of microorganisms from specified pharmaceutical water systems and low-nutrient environments

All products in the selective media range are supplied as 90 mm plates in units of 60 (6 stacks with 10 plates each).

Ready-to-Use 90 mm Selective Agar Plates

PMM’s selective media are supplied as ready-to-use 90 mm plates, eliminating the need for laboratory preparation of the agar before use.

The plates are:

• 90 mm format
• Single-bagged
• Non-irradiated
• Supplied in units of 60
• Available for pharmaceutical, industrial, laboratory, and research applications

The plates feature a dual position locking lid system for optimised incubation in both aerobic and anaerobic conditions.

Cetrimide Agar for Pseudomonas aeruginosa Testing

Cetrimide Agar 90 mm CS — Item 450.0060

PMM Cetrimide Agar is intended for use in the examination of non-sterile products and testing for Pseudomonas aeruginosa in the pharmaceutical industry.

The specified application involves preparation of the product sample, enrichment in Tryptic Soy Broth (TSB), followed by subculture onto Cetrimide Agar. After incubation, the growth of colonies indicates the presence of P. aeruginosa. Where colonies are detected, an identification test is required to establish the presence or absence of P. aeruginosa.

Application: Test for Pseudomonas aeruginosa
Format: 90 mm CS plate
Pack size: 60 plates
Item: 450.0060

Burkholderia cepacia Complex Selective Agar

BCSSA 90 mm CS — Item 451.0060

PMM Burkholderia cepacia Complex Selective Agar is intended for the examination of non-sterile products and testing for Burkholderia cepacia complex.

The specified application includes sample preparation followed by incubation in Soybean–Casein Digest Broth or an appropriate dilution determined during method suitability. The sample is subsequently subcultured onto Burkholderia cepacia Complex Selective Agar. Growth of colonies indicates the presence of B. cepacia complex, with identification testing required to confirm any detected growth.

Application: Test for Burkholderia cepacia complex
Format: 90 mm CS plate
Pack size: 60 plates
Article: 451.0060

Mannitol Salt Agar for Staphylococcus aureus Testing

Mannitol Salt Agar 90 mm CS — Item 455.0060

PMM Mannitol Salt Agar is designed for the examination of non-sterile products and testing for Staphylococcus aureus.

Following sample preparation and enrichment in Tryptic Soy Broth, an aliquot is subcultured onto Mannitol Salt Agar. Colonies surrounded by a yellow/white zone indicate the presence of S. aureus. Where such colonies are detected, an identification test is required to establish the presence or absence of S. aureus.

Application: Test for Staphylococcus aureus
Format: 90 mm CS plate
Pack size: 60 plates
Item: 455.0060

MacConkey Agar for Escherichia coli Testing

MacConkey Agar 90 mm CS — Item 460.0060

PMM MacConkey Agar is intended for the examination of non-sterile products and testing for Escherichia coli.

The specified application includes sample preparation, enrichment in TSB, transfer to MacConkey Broth, and subsequent subculture onto MacConkey Agar. Growth of colonies indicates the presence of E. coli. Any detected colonies require identification testing to establish the presence or absence of E. coli.

Application: Test for Escherichia coli
Format: 90 mm CS plate
Pack size: 60 plates
Item: 460.0060

VRBD Agar for Bile-Tolerant Gram-Negative Bacteria

VRBD Agar 90 mm CS — Item 465.0060

PMM VRBD Agar is intended for the examination of non-sterile products and testing for bile-tolerant Gram-negative bacteria.

The specified application includes sample preparation and incubation in Tryptic Soy Broth, followed by transfer to Mossel Broth. An aliquot is subsequently subcultured onto VRBD Agar. Growth of colonies indicates the presence of bile-tolerant Gram-negative bacteria.

Application: Test for bile-tolerant Gram-negative bacteria
Format: 90 mm CS plate
Pack size: 60 plates
Item: 465.0060

XLD Agar for Salmonella spp. Testing

XLD Agar 90 mm CS — Item 470.0060

PMM XLD Agar is intended for the examination of non-sterile products and testing for Salmonella spp.

The specified application involves sample preparation and enrichment in Tryptic Soy Broth, followed by transfer to Rappaport-Vassiliadis Broth. An aliquot is then subcultured onto XLD Agar. Growth of reddish or red colonies, with or without a black centre, indicates the presence of Salmonella. Where such colonies are detected, an identification test is required to establish the presence or absence of Salmonella.

Application: Test for Salmonella spp.
Format: 90 mm CS plate
Pack size: 60 plates
Item: 470.0060

R2A Agar for Pharmaceutical Water Microbiology

R2A Agar 90 mm CS — Item 490.0060

PMM R2A Agar is intended for the detection of microorganisms from water for injections in bulk, highly purified water, and purified water in bulk. It can also be used for the detection of aerobic, heterotrophic microorganisms from low-nutrient environments.

The product is suitable for direct inoculation or application of filters, providing a ready-to-use plate format for relevant microbiological testing applications.

Applications:

• Detection of microorganisms from water for injections in bulk
• Detection of microorganisms from highly purified water
• Detection of microorganisms from purified water in bulk
• Direct inoculation or application of filters
• Detection of aerobic, heterotrophic microorganisms from low-nutrient environments

Format: 90 mm CS plate
Pack size: 60 plates
Item: 490.0060

Plate Design for Practical Laboratory Handling

PMM selective media plates incorporate a locking lid system with two positions to support different incubation requirements.

The closed position provides ideal incubation conditions for aerobic microorganisms, thereby limiting agar dehydration.  For longer aerobic incubation periods, the closed position is recommended. The vent position is ideal for anaerobic incubations, as it allows an easy and effective removal of oxygen under anaerobic incubation conditions. This position should be used for incubation in anaerobic incubator or anaerobic jar.

Certificate of Analysis for Every Batch

Every batch of PMM products can be obtained with a Certificate of Analysis (CoA).

Batch-specific documentation provides customers with access to product information associated with the individual batch and supports the documentation requirements of laboratory, industrial, research, and pharmaceutical workflows.

Selective Media in Plates for Pharmaceutical Testing at a Glance

Product line: Selective Media in Plates
Application: Pharmaceutical industry; industrial, laboratory and research applications
Primary applications: Examination of non-sterile products and testing for specified microorganisms
Plate format: 90 mm
Packaging: Single-bagged
Irradiation: Non-irradiated
Pack size: 60 plates (6 stacks with 10 plates each)
Documentation: TDS and MSDS available; CoA available for every batch across all PMM products
Media range: Cetrimide, BCSSA, Mannitol Salt, MacConkey, VRBD, XLD and R2A Agar

A Broad Portfolio for Pharmaceutical Microbiological Testing

PMM’s Selective Media in Plates portfolio provides ready-to-use 90 mm agar plates for a range of pharmaceutical microbiological testing applications. From specified microorganism testing for Pseudomonas aeruginosa, Burkholderia cepacia complex, Staphylococcus aureus, Escherichia coli, and Salmonella spp. to testing for bile-tolerant Gram-negative bacteria and microorganisms in pharmaceutical water systems, the portfolio covers diverse laboratory requirements.

With single-bagged, non-irradiated plates, defined applications, batch-specific CoA availability, and supporting technical and safety documentation, PMM provides pharmaceutical laboratories with a practical range of selective culture media for routine microbiological testing.

For more information, please contact PMM today.

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