Human CTGF Colorimetric Sandwich ELISA Kit

The OmniKine™ Human CTGF ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that allows for the detection and quantification of human connective tissue growth factor (CTGF) in cell lysates, serum and plasma samples.

This kit provides researchers with a powerful tool to study the expression, function, and regulation of Human CTGF in various biological systems and conditions.

The OmniKine™ Human CTGF ELISA Kit

The OmniKine™ Human CTGF Colorimetric Sandwich ELISA Kit offered by Assay Biotechnology allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CTGF proteins within the range of 63-4000 pg/ml. This kit provides all the essential components required for quantitative determination, ensuring precise and reliable results for Human CTGF concentration analysis.

Human Connective Tissue Growth Factor (CTGF) is a 349 amino acid growth factor encoded by the CTGF gene located at locus 6q23.1 on chromosome 6. It is characterized as a major connective tissue mitoattractant secreted by vascular endothelial cells. CTGF promotes proliferation and differentiation of chondrocytes, mediates cell adhesion in various cell types, and enhances fibroblast growth factor-induced DNA synthesis. The expression of CTGF is mainly observed in bone marrow and thymic cells.

Excellent sensitivity and dynamic range for measuring Human CTGF concentrations

The Human CTGF ELISA Kit offers excellent sensitivity and a wide dynamic range for measuring Human CTGF concentrations. With a dynamic range of 63-4000 pg/ml, this ELISA kit provides precise and accurate quantification of Human CTGF levels. The assay has been extensively validated for sensitivity, linearity, recovery, precision, and specificity using various biological samples, ensuring reliable and reproducible results.

Detects both recombinant and naturally produced human CTGF proteins

The OmniKine™ Human CTGF ELISA Kit is highly sensitive and specific for the detection of Human CTGF. It is capable of recognizing both recombinant and naturally produced Human CTGF proteins. Rigorous testing has been conducted to evaluate cross-reactivity and interference. The results demonstrate that the kit does not exhibit significant cross-reactivity or interference with other related antigens, such as BMP-4, CTGFL/WISP-2, CYR61, IGF-I, IGF-II, IGF-BP1, NOV, TGF-beta, WISP-1, and WISP-3, at a concentration of 50 ng/ml. This ensures accurate and reliable measurement of Human CTGF levels in various biological samples.

Ready-to-use: The OmniKine™ Human CTGF ELISA Kit includes all necessary components

The assay can be completed within 4.5 hours, providing researchers with an efficient workflow and enabling timely data generation. The kit includes all the necessary components, including microstrips coated with capture antibody, lyophilized protein standard, biotinylated detection antibody, Streptavidin-HRP conjugate, wash buffer, assay diluents, ready-to-use substrate, stop solution, adhesive plate sealers, and a technical manual.

The OmniKine™ Human CTGF ELISA Kit also includes a detailed protocol and safety datasheet. These documents provide key information on specifications, quality control procedures, and performance characteristics. This ensures that researchers have all the necessary information to successfully perform the assay and interpret the results.

Human CTGF recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human CTGF were spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for CTGF. Each known sample concentration was assayed in triplicate. The OmniKine™ Human CTGF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CTGF proteins within the range of 63-4000 pg/ml.

The Human CTGF (Catalog No: OK-0109) OmniKine™ ELISA Kit by Assay Biotechnology is a valuable tool for researchers studying the expression, function, and regulation of Human CTGF. With its excellent sensitivity, wide dynamic range, compatibility with various sample types, and comprehensive set of components, this ELISA kit enables accurate and high-quality measurements of Human CTGF concentrations in different experimental settings.

Specifications Summary – OmniKine™ Human CTGF Colorimetric Sandwich ELISA Kit

Assay Biotechnology – Catalog No: OK-0109

  • Reactivity: Human
  • Assay Type: Sandwich ELISA
  • Format: 1 plate: 12 x 8-Well Microstrips
  • Dynamic Range: 63-4000 pg/ml

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