The OmniKine™ Rat IP-10 ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that allows for the detection and quantification of rat interferon gamma-induced protein 10 (IP-10) in supernatants, sera and plasma samples.
OmniKine™ Rat IP-10 Colorimetric Sandwich ELISA Kit
The OmniKine™ Rat IP-10 Colorimetric Sandwich ELISA Kit offered by Assay Biotechnology is a comprehensive solution designed to accurately measure the levels of rat interferon gamma-induced protein 10 (IP-10) in various experimental samples, including supernatants, sera, and plasma. This versatility allows researchers to analyze IP-10 levels in a wide range of experimental samples relevant to their specific research interests. The kit provides all the essential components required for quantitative determination, ensuring precise and reliable results for IP-10 concentration analysis.
IP-10 is a member of the C-X-C motif chemokine family and is induced by interferon gamma and other inflammatory stimuli. It plays crucial roles in immune and inflammatory responses, including chemotaxis, angiogenesis, cell proliferation, and antiviral activity. Furthermore, IP-10 is involved in T-cell effector function and development, and its expression can be modulated by oncogenic Ras and serum growth factors.
Excellent sensitivity and dynamic range for measuring IP-10 concentrations
The Rat IP-10 OmniKine™ ELISA Kit provides researchers with the capability to detect and quantify both endogenous levels of natural and recombinant Rat IP-10 proteins within a wide dynamic range of 4-1000 pg/ml. This excellent sensitivity range ensures accurate and precise measurement of IP-10 concentrations, enabling researchers to study the expression, function, and regulation of Rat IP-10 in various biological systems and conditions.
The assay utilizes a colorimetric signal that is directly proportional to the amount of rat IP-10 present in the sample. The absorbance can be measured at 450 nm using a spectrophotometer, providing accurate results for IP-10 concentration analysis.
Detects both recombinant and naturally produced Rat IP-10 proteins
The OmniKine™ Rat IP-10 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IP-10 proteins within the range of 4-1000 pg/ml. It is capable of recognizing both recombinant and naturally produced Rat IP-10 proteins. Cross-reactivity testing was conducted at 50 ng/ml, and the kit exhibited less than 2% cross reactivity with Murine IP-10. Furthermore, it did not exhibit significant cross reactivity or interference with the listed human, murine, and rat antigens at the tested concentrations.
Ready-to-use: OmniKine™ Rat IP-10 Colorimetric Sandwich ELISA Kit includes all necessary components
The OmniKine™ Rat IP-10 ELISA Kit includes all the necessary components to perform the assay. The Rat IP-10 OmniKine™ ELISA Kit includes the following components per kit: 12 x 8-Well Microstrips coated with Capture Antibody, Lyophilized Protein Standard, Lyophilized Biotinylated Detection Antibody, 400x Streptavidin-HRP B (30 μl), Wash Buffer (15x, 50 ml), Assay Diluent 45HB (15 ml), Assay Diluent 1TD (50 ml), Ready-to-Use Substrate (12 ml), Stop Solution (12 ml), Adhesive Plate Sealers (2 sheets), Technical Manual (1 manual). After the first use, the Microstrips coated with Capture Antibody can be stored for 1 month at 4°C.
The Rat IP-10 OmniKine™ ELISA Kit also includes a detailed protocol and safety datasheet. These documents provide important information about product specifications, quality control procedures, and performance characteristics. This ensures that researchers have all the necessary information to successfully perform the assay and interpret the results.
The OmniKine™ Rat IP-10 Colorimetric Sandwich ELISA Kit by Assay Biotechnology is a valuable tool for researchers studying the expression, function, and regulation of rat IP-10. With its ease of use, reliability, sensitivity, and wide range of applications, this ELISA kit enables accurate and high-quality measurements of IP-10 concentrations in various biological samples.
Specifications Summary – OmniKine™ Rat IP-10 Colorimetric Sandwich ELISA Kit
Assay Biotechnology – Catalog No: OK-0209
- Reactivity: Rat
- Assay Type: Sandwich ELISA
- Format: 1 plate: 12 x 8-Well Microstrips
- Dynamic Range: 16-1000 pg/ml
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