The eIF2α (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit is an efficient and sensitive assay, designed for detecting the protein expression of Phospho-eIF2α (Ser51) in cells without the need for cell lysate.
This kit offers the advantages of convenience and high throughput capabilities. It is an ideal tool for assessing relative levels of Phospho-eIF2α (Ser51) in cultured cells.
Additionally, it serves as a valuable tool for evaluating the impact of various treatments, inhibitors (such as siRNA or chemicals), or activators on Phospho-eIF2α (Ser51) levels.
CytoGlow™ eIF2α (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit
The eIF2α (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit offered by Assay Biotechnology is an innovative and user-friendly assay kit designed to detect and quantify the expression profile of Phospho-eIF2α (Ser51) protein in cells. This kit offers a range of advantages, including its convenience, high throughput capacity, and exceptional sensitivity. Moreover, it eliminates the need for lysate preparation, streamlining the experimental process and saving valuable time for researchers.
The primary application of this kit lies in its ability to precisely monitor the phosphorylation status of eIF2α at Ser51 and evaluate its expression levels in cultured cells. This capability empowers researchers to investigate the functional significance of eIF2α phosphorylation in different cellular contexts.
Beyond quantification, the Phospho-eIF2α (Ser51) Colorimetric Cell-Based ELISA Kit is also ideal for screening the effects of diverse treatments, inhibitors (such as siRNA or chemicals), or activators on eIF2α phosphorylation. This screening functionality allows for comprehensive investigations into the regulatory mechanisms governing eIF2α signaling and its impact on cellular processes.
To ensure the utmost specificity and reliability, the kit includes highly specific antibodies. The Anti-eIF2α (Phospho-Ser51) Antibody, a rabbit polyclonal antibody, has undergone thorough testing on Western Blots, revealing a single protein band detected in the presence of the immunogen peptide. This confirms the antibody’s precision in recognizing eIF2α phosphorylated at Ser51.
Excellent specificity for phosphorylated eIF2α at Ser51
The Phospho-eIF2α (Ser51) Colorimetric Cell-Based ELISA Kit demonstrates remarkable specificity, as validated through various assays. The Anti-eIF2α (Phospho-Ser51) Antibody, a rabbit polyclonal antibody utilized in the kit, was subjected to Western Blot analysis. This analysis showed the detection of a single protein band in extracts from K562 cells treated with IFN-alpha. Moreover, this protein band’s specificity was further confirmed, as it could be effectively blocked by the synthesized immunogen peptide. Similarly, in an ELISA assay, the Anti-eIF2α (Phospho-Ser51) Antibody exhibited high specificity for the phospho-peptide compared to the non-phospho peptide counterpart. These findings substantiate the antibody’s ability to selectively target and recognize phosphorylated eIF2α at Ser51.
Furthermore, the Anti-eIF2α Antibody, another rabbit polyclonal antibody, demonstrated specificity through Western Blot analysis, where a single protein band was detected in rat lung extracts. The specificity of the Anti-GAPDH Antibody, a mouse monoclonal antibody, was also validated on Western Blots using tissue lysates from human, mouse, and rat, showing detection of a single protein band in all three lysates. The stringent antibody specificity tests ensure that the Phospho-eIF2α (Ser51) Colorimetric Cell-Based ELISA Kit delivers reliable and accurate results, enabling researchers to confidently investigate the phosphorylation status of eIF2α and its potential implications in cellular processes.
Ready-to-use: The eIF2α (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit Includes All Necessary Components
The eIF2α (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit comes with all the necessary reagents for successful experimentation. The package includes a 96-well cell culture clear-bottom microplate, 10x TBS, quenching buffer, blocking buffer, 15x wash buffer, primary antibodies specific for Phospho-eIF2α (Ser51), HRP-conjugated secondary antibodies, substrate solution, stop solution, crystal violet solution, and adhesive plate seals.
For optimal results, the kit should be stored at 4°C upon receipt. The unopened kit remains stable for up to six months from the date of shipment when stored under these conditions. Additionally, diluted Anti-Phospho-eIF2α (Ser51) Antibody, eIF2α Antibody, and diluted Anti-GAPDH Antibody can each be stored at 4°C for up to two weeks. HRP-conjugated secondary antibodies are stable at 4°C for up to six months. The SDS Solution and Quenching Buffer should be stored at room temperature or warmed to room temperature if initially stored at 4°C.
The eIF2α (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit also includes a detailed protocol and safety datasheet. These documents provide information about product specifications, quality control procedures, preparation, and performance characteristics. This ensures that researchers have all the required information to successfully perform the assay and interpret the results.
With its exceptional convenience and precision, the eIF2α (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit is a valuable tool for researchers seeking to explore the intricacies of eIF2α signaling and its impact on cellular responses. Whether investigating cellular stress responses, analyzing the effects of various treatments, or studying the regulation of translation initiation, this kit enables researchers to gain valuable insights and accelerate their scientific discoveries.
Specifications Summary – eIF2α (Phospho-Ser51) Colorimetric Cell-Based ELISA Kit
Assay Biotechnology – Catalog No: CBP1538
- Reactivity: H:S52,M:S52,R:S52
- Assay Type: Cell-Based ELISA
- Format: 2 Plates: TC 96-Well Plates
- Dynamic Range: At least 5000 cells/well
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