ACK1 (Phospho-Tyr284) Cell-Based ELISA Kit

The ACK1 (Phospho-Tyr284) Cell-Based ELISA Kit offers a user-friendly, high throughput, and sensitive assay solution for efficiently detecting ACK1 protein phosphorylation and expression in cells.

This kit is designed to work without the need for lysate preparation, making it convenient to use. Its capabilities allow for the quantification of phosphorylated ACK1 in cultured cells, enabling researchers to assess the impact of different treatments, inhibitors (e.g., siRNA or chemicals), or activators on ACK1 phosphorylation levels.

CytoGlow™ ACK1 (Phospho-Tyr284) Cell-Based ELISA Kit

The Phospho-ACK1 (Tyr284) Colorimetric Cell-Based ELISA Kit offered by Assay Biotechnology is a cutting-edge and highly efficient assay kit designed to detect and quantify the phosphorylation status of ACK1 protein at Tyr284 in various cell types. This kit offers a range of advantages, including its convenience, high throughput capacity, and remarkable sensitivity. Moreover, it does not require lysate preparation, streamlining the process and saving valuable time for researchers.

The key application of this kit lies in its ability to monitor ACK1 protein phosphorylation and expression profiles within cells. Researchers can precisely measure the relative amounts of phosphorylated ACK1 in cultured cells, enabling detailed investigations into the functional implications of ACK1 activation in different experimental conditions.

One of the key features of the Phospho-ACK1 (Tyr284) Cell-Based ELISA Kit is its capability to facilitate the screening of various treatments, inhibitors (such as siRNA or chemicals), or activators for their effects on ACK1 phosphorylation. This screening functionality opens up a world of possibilities for studying the regulation of ACK1 signaling pathways and understanding the impact of specific agents on their activity.

Excellent specificity for ACK1 phosphorylated at Tyr284

The specificity of the kit’s antibodies is of paramount importance. The Anti-ACK1 (Phospho-Tyr284) Antibody used in the kit has been thoroughly tested on Western Blots to ensure its accuracy and reliability. The data from these tests show that a single protein band was detected, and this protein band could be effectively blocked by the synthesized immunogen peptide, affirming the antibody’s specificity for ACK1 phosphorylated at Tyr284.

Similarly, the Anti-ACK1 Antibody and the Anti-GAPDH Antibody (a mouse monoclonal antibody) employed in the kit were also tested for specificity on Western Blots. In both cases, a single protein band was detected, further confirming the accuracy of the kit’s antibodies.

Ready-to-use: The ACK1 (Phospho-Tyr284) Cell-Based ELISA Kit Includes All Necessary Components

The Phospho-ACK1 (Tyr284) Colorimetric Cell-Based ELISA Kit comes complete with all the necessary reagents for successful experimentation. The package includes a 96-well cell culture clear-bottom microplate, 10x TBS, quenching buffer, blocking buffer, 15x wash buffer, primary antibodies (Anti-Phospho-ACK1 (Tyr284) Antibody, Anti-ACK1 Antibody, and Anti-GAPDH Antibody), HRP-conjugated secondary antibodies (Anti-Rabbit IgG and Anti-Mouse IgG), primary antibody diluent, ready-to-use substrate, stop solution, crystal violet solution, SDS solution, and adhesive plate seals.

To ensure optimal results and reliability, the kit should be stored at 4°C upon receipt. The unopened kit will remain stable for up to six months from the date of shipment under these storage conditions. Additionally, diluted Anti-Phospho-ACK1 (Tyr284) Antibody, ACK1 Antibody, and diluted Anti-GAPDH Antibody can each be stored at 4°C for up to two weeks. The HRP-conjugated secondary antibodies will remain stable at 4°C for up to six months. The SDS Solution and Quenching Buffer should be stored at room temperature or warmed to room temperature if initially stored at 4°C.

The Phospho-ACK1 (Tyr284) Colorimetric Cell-Based ELISA Kit also includes a detailed protocol and safety datasheet. These documents provide information about product specifications, quality control procedures, preparation, and performance characteristics. This ensures that researchers have all the required information to successfully perform the assay and interpret the results.

Enzyme-Linked Immunosorbent Assay (ELISA) for
immunogen phosphor-peptide (left) and non-phospho peptide (right),
using Anti-ACK1 (Phospho-Tyr284) Antibody.
Western blot analysis of tissue lysates from human (1), mouse
(2) and rat (3).
Cell-Based Phospho Western Blot
Western blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30′, using ACK1 (Phospho-Tyr284) Antibody.
Cell-Based Non-Phospho Western Blot
WB analysis of the lysates from COLO205 cells

The Phospho-ACK1 (Tyr284) Colorimetric Cell-Based ELISA Kit offers a comprehensive and reliable tool for researchers studying the regulation and function of ACK1 phosphorylation. Its user-friendly features, precise antibody specificity, and comprehensive reagent package make it an indispensable asset in the field of cell-based ELISA assays. Whether investigating cellular signaling pathways or evaluating the effects of various agents on ACK1 activity, this kit empowers researchers to gain valuable insights and accelerate their scientific discoveries.

Specifications Summary – ACK1 (Phospho-Tyr284) Colorimetric Cell-Based ELISA

Assay Biotechnology – Catalog No: CBP1236

  • Reactivity: H:Y284, M:Y284
  • Assay Type: Cell-Based ELISA
  • Format: 2 Plates: TC 96-Well Plates
  • Dynamic Range: At least 5000 cells/well

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