Synuclein-α (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit

The Synuclein-α (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit is a user-friendly and highly sensitive assay designed to detect the protein expression profile of Phospho-Synuclein-α (Ser129) in cells without the need for cell lysate.

With its convenience and high throughput capabilities, this kit is an excellent tool for quantifying the relative levels of Phospho-Synuclein-α (Ser129) in cultured cells.

Moreover, it facilitates screening for the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Phospho-Synuclein-α (Ser129) levels.

CytoGlow™ Synuclein-α (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit

The Synuclein-α (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit offered by Assay Biotechnology is an advanced and reliable assay designed to study the phosphorylation status and expression profile of Synuclein-α protein in cells. This kit offers several advantages, including its ease of use, high throughput capabilities, and sensitivity, without the need for lysate preparation, streamlining the experimental process.

Researchers can utilize this kit to accurately measure the relative amounts of phosphorylated Synuclein-α in cultured cells. Additionally, it allows for screening the effects of various treatments, inhibitors (e.g., siRNA or chemicals), or activators on Synuclein-α phosphorylation. Understanding these regulatory mechanisms can shed light on the role of Synuclein-α in cellular processes and potential implications in various diseases, including neurodegenerative disorders.

Excellent specificity for phosphorylated Synuclein-α at Ser129

The specificity of the Anti-Synuclein-α (Phospho-Ser129) Antibody in the Synuclein-α (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit is validated through various experimental techniques. Firstly, the antibody was subjected to Western Blot analysis. The Anti-Synuclein-α (Phospho-Ser129) Antibody detected a single protein band in extracts from mouse brain, specifically targeting phosphorylated Synuclein-α at Ser129. Importantly, this protein band was effectively blocked by the synthesized immunogen peptide, confirming the antibody’s specificity for the phospho-Ser129 epitope.

Furthermore, an ELISA assay was performed to assess the antibody’s specificity more comprehensively. The results revealed a clear distinction between the immunogen phospho-peptide and the non-phospho peptide counterpart, with the Anti-Synuclein-α (Phospho-Ser129) Antibody displaying high specificity for the phospho-peptide. This specificity ensures accurate and reliable measurements of phosphorylated Synuclein-α levels in cellular samples.

In addition to the Anti-Synuclein-α (Phospho-Ser129) Antibody, the Anti-Synuclein-α Antibody was also tested for specificity on Western Blots. Similar to the phospho-specific antibody, the non-phospho-specific antibody also detected a single protein band. Importantly, this band was blocked by the synthesized immunogen peptide, further confirming the specificity of both antibodies for their respective targets.

As a control, the Anti-GAPDH Antibody, a mouse monoclonal antibody, was included in the Western Blot experiments using tissue lysates from human, mouse, and rat. The data demonstrated the specificity of the Anti-GAPDH Antibody, with a single protein band detected from all three lysates.

Ready-to-use: The Synuclein-α (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit Includes All Necessary Components

Assay Biotech’s Synuclein-α (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit includes all necessary reagents for easy and efficient experimentation. The package comprises a 96-well cell culture clear-bottom microplate, 10x TBS, quenching buffer, blocking buffer, 15x wash buffer, primary antibodies specific for Phospho-Synuclein-α (Ser129), HRP-conjugated secondary antibodies, substrate solution, stop solution, crystal violet solution, and adhesive plate seals.

Proper storage is essential to ensure accurate results. Upon receipt, the kit should be stored at 4°C. The unopened kit remains stable for up to six months from the date of shipment when stored under these conditions. Diluted Anti-Phospho-Synuclein-α (Ser129) Antibody, Synuclein-α Antibody, and diluted Anti-GAPDH Antibody can each be stored at 4°C for up to two weeks. HRP-conjugated secondary antibodies are stable at 4°C for up to six months. The SDS Solution and Quenching Buffer should be stored at room temperature or warmed to room temperature if initially stored at 4°C.

The Synuclein-α (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit also includes a detailed protocol and safety datasheet. These documents provide information about product specifications, quality control procedures, preparation, and performance characteristics. This ensures that researchers have all the required information to successfully perform the assay and interpret the results.

The Synuclein-α (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit serves as a valuable tool for researchers exploring the phosphorylation status and expression patterns of Synuclein-α in cellular contexts. It’s high specificity and ease of use make it a reliable choice for investigating the intricate role of Synuclein-α in cellular signaling and potential implications in human health and disease. Whether studying neurodegeneration or investigating novel therapeutic approaches, this kit empowers researchers to delve deeper into the complexities of Synuclein-α biology.

Specifications Summary – Synuclein-α (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit

Assay Biotechnology – Catalog No: CBP1221

• Reactivity: H:S129,M:S129,R:S129
• Assay Type: Cell-Based ELISA
• Format: 2 Plates: TC 96-Well Plates
• Dynamic Range: At least 5000 cells/well

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