The COL1A2 Antibody from Assay Biotechnology is a highly specific polyclonal antibody designed to target the pro-alpha2 chain of type I collagen (COL1A2). This antibody is capable of detecting COL1A2 protein in human, mouse, and rat tissues and cells. It has been extensively validated for various applications, including Western blotting (WB), immunohistochemistry on paraffin-embedded sections (IHC-p), immunofluorescence on paraffin sections (IF/ICC), and enzyme-linked immunosorbent assay (ELISA).
The antibody exhibits exceptional specificity in recognizing the pro-alpha2 chain of type I collagen, enabling researchers to investigate the expression and localization of COL1A2 in different samples and experimental settings.
The COL1A2 Antibody is a polyclonal rabbit antibody that targets the pro-alpha2 chain of type I collagen (COL1A2), a protein that forms the triple helix of type I collagen, the most abundant type of collagen in the human body. Type I collagen is a fibril-forming collagen that provides strength and flexibility to connective tissues such as bone, cornea, dermis and tendon. COL1A2 is encoded by the COL1A2 gene, which can undergo mutations that affect the synthesis, stability and assembly of type I collagen, leading to various connective tissue disorders such as osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis and atypical Marfan syndrome. The antibody can be used for different applications to study the expression and localization of COL1A2 in human, mouse and rat samples such as WB, IHC-p, IF/ICC and ELISA.
Reactivity in Human, Mouse, and Rat Samples
The COL1A2 Antibody is designed to detect and localize COL1A2 protein in a variety of species, including human, mouse, and rat. This broad reactivity allows researchers to investigate collagen-related processes and disorders across different model organisms, facilitating translational research and comparative studies. It has been extensively validated WB, IHC-p, IF/ICC and ELISA applications.
High Specificity – Detects endogenous levels of COL1A2 protein
The antibody has been extensively validated to ensure high specificity in detecting the pro-alpha2 chain of type I collagen (COL1A2). This specificity minimizes the potential for cross-reactivity with other proteins, providing accurate and reliable results. By selectively targeting COL1A2, researchers can accurately study its expression, localization, and biological functions in various biological samples.
The ability to detect endogenous levels of COL1A2 protein is crucial for understanding its physiological role and its involvement in connective tissue disorders. It allows researchers to investigate the native expression patterns and dynamic changes of COL1A2 in different tissues, developmental stages, and disease conditions. This information can provide valuable insights into the mechanisms underlying collagen-related disorders, facilitate the identification of potential therapeutic targets, and contribute to the development of diagnostic tools.
The specificity of the COL1A2 Polyclonal Antibody in detecting endogenous levels of COL1A2 protein provides researchers with a powerful tool to study its expression, localization, and functions in various biological contexts. This specificity enhances the accuracy, reliability, and relevance of research findings, leading to a better understanding of collagen-related disorders and potential therapeutic interventions.
Purity and Concentration
The COL1A2 antibody is affinity-purified from rabbit antiserum by affinity-chromatography utilising epitope-specific immunogen derived from the first 50 amino acids of human COL1A2 protein. This ensures enhanced specificity and reduces nonspecific binding. The COL1A2 antibody has a concentration of 1 mg/ml and is supplied in a convenient liquid formulation containing 50% glycerol, 0.5% bovine serum albumin (BSA), and 0.02% sodium azide. This formulation enhances the stability and longevity of the antibody, making it suitable for long-term storage and repeated use.
Storage and Stability
For long-term stability, it is recommended to store the antibody at -20°C, and it has a shelf life of up to one year under appropriate storage conditions. The antibody is provided with a comprehensive Product Datasheet and Safety Datasheet that provide detailed information regarding product specifications, experimental protocols, and quality control.
Performance and Reliability in Experimental Settings
Application data showcases the performance and reliability of the COL1A2 Antibody in various experiments and sample types. These data demonstrate the high sensitivity and specificity of the antibody. These include immunohistochemistry analysis of human breast carcinoma tissue, immunofluorescence analysis of NIH/3T3 cells, and Western blot analysis of various cell lysates.
The COL1A2 Antibody from Assay Biotechnology offers highly specific detection of type I collagen through its affinity for the pro-alpha2 chain of COL1A2. With its broad reactivity in human, mouse, and rat samples, along with its high specificity, versatility, and comprehensive data support, this antibody serves as a valuable tool for researchers studying collagen-related disorders and investigating the expression and localization of COL1A2 in diverse biological contexts.
Specifications Summary – COL1A2 Antibody
Assay Biotechnology – Catalog No: C0154
- Reactivity: Human, Mouse, Rat
- Applications: WB, IHC-p, IF/ICC, ELISA
- Modification: Unmodified/Total
- Source: Polyclonal Rabbit
- Storage and Stability: -20°C/1 year
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