Akt (Phospho-Thr308) Antibody

The Akt (Phospho-Thr308) Antibody from Assay Biotechnology is a highly specific polyclonal antibody designed to target the phosphorylated form of Akt at Thr308. This antibody is capable of detecting Akt (Phospho-Thr308) in human, mouse, rat, and monkey tissues and cells. It is validated for WB, IHC-p, IF(paraffin section), and ELISA applications.

The Phospho-Akt (T308) Polyclonal Antibody specifically detects endogenous levels of Akt protein only when phosphorylated at T308.

Akt (Phospho-Thr308) Antibody

Assay Biotech’s Akt (Phospho-Thr308) Antibody is a highly specific and reliable polyclonal rabbit antibody designed to target the phosphorylated form of Akt at Thr308. Akt, also known as protein kinase B (PKB), is a serine-threonine protein kinase that plays a crucial role in mediating a wide range of cellular processes, including survival, growth, proliferation, and metabolism, in response to extracellular signals.

Activation of Akt occurs through phosphorylation events, where phosphatidylinositol 3-kinase (PI3K) activates Akt and phosphoinositide-dependent kinase 1 (PDK1) phosphorylates Akt at Thr308. Subsequently, the mammalian target of rapamycin complex 2 (mTORC2) phosphorylates Akt at Ser473. The phosphorylation of Akt at Thr308 is critical for its full activation and proper functioning within the cell.

Immunodetection in Various Samples and Applications

The Akt (Phospho-Thr308) Antibody is specifically designed to detect and quantify the phosphorylation status and activity of Akt in various biological samples and applications. It has undergone extensive validation for Western blotting, immunohistochemistry, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). This antibody is capable of detecting Akt (Phospho-Thr308) in human, mouse, rat, and monkey tissues and cells, making it suitable for a wide range of research purposes.

High Specificity – Only Detects the Phosphorylated form of Akt at Thr308

The Akt (Phospho-Thr308) Polyclonal Antibody has been specifically developed to recognize Akt protein only when it is phosphorylated at T308. This means that the antibody binds selectively to the activated form of Akt, enabling researchers to detect and study the phosphorylated state of Akt in different experimental settings. By specifically targeting the phosphorylated Akt at T308, the antibody allows researchers to assess the activation status of Akt and investigate its role in signaling pathways and cellular functions.

The antibody does not recognize the non-phosphorylated form of Akt or Akt phosphorylated at other sites. Therefore, it provides a valuable tool for studying the specific phosphorylation event at T308 and its functional implications in Akt signaling. By detecting endogenous levels of phosphorylated Akt at T308, the antibody enables researchers to gain insights into the regulation and activity of Akt in various cellular processes and disease contexts.

Purity and Concentration

The antibody is produced from rabbit antiserum and purified using affinity chromatography, utilizing a synthetic peptide derived from human Akt around the phosphorylation site of Thr308 as the immunogen. With a concentration of 1 mg/ml, the antibody exhibits a molecular weight of approximately 60 kDa. It is supplied in a liquid formulation in phosphate-buffered saline (PBS) containing 50% glycerol, 0.5% bovine serum albumin (BSA), and 0.02% sodium azide as a preservative.

Storage and Stability

For long-term stability, it is recommended to store the antibody at -20°C, and it has a shelf life of up to one year under appropriate storage conditions. The antibody is accompanied by a comprehensive Product Datasheet and Safety Datasheet that provide detailed information regarding product specifications, experimental protocols, and quality control. Additionally, a certificate of analysis is provided to confirm the purity and specificity of the antibody.

Performance and Reliability in Experimental Settings

Application data showcases the performance and reliability of the Akt (Phospho-Thr308) Antibody in various experiments and sample types. These data demonstrate the high sensitivity and specificity of the antibody, reinforcing its suitability for studying Akt signaling and function in diverse biological systems.

The Akt (Phospho-Thr308) Antibody from Assay Biotechnology is a valuable tool for researchers interested in investigating the phosphorylation status and activity of Akt in various cellular contexts. Its specificity, extensive validation, and wide reactivity across species make it an excellent resource for understanding the intricate role of Akt in cellular signaling pathways.

Specifications Summary – Akt (Phospho-Thr308) Antibody

Assay Biotechnology – Catalog No: A7005

  • Reactivity: Human, Mouse, Rat, Monkey
  • Applications: WB, IHC-p, IF(paraffin section), ELISA
  • Modification: Phospho Specific
  • Source: Polyclonal Rabbit
  • Storage: and Stability-20°C/1 year

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