Ready-to-use HEK293 Cell-based In Vitro Plates

ReadyCell has developed an advanced range of ready-to-use HEK293 Cell-based In Vitro Plates for studying uptake drug-transporter interactions in preclinical testing and drug discovery.

The PreadyTake range of pre-plated HEK293 cell-based models provides a cutting-edge tool to investigate drug renal clearance and biliary excretion. The PreadyTake MATE1, PreadyTake OCT2, and PreadyTake OATP1B3 subproducts of the PreadyTake line are developed to cater to different research needs for drug-transporter interaction studies according to regulatory recommendations.

HEK293 Cell-based Plates for Drug-Transporter Assays

ReadyCell’s unique in vitro HEK293 cell-based plates, featuring elevated expression of uptake transporters, provide a cutting-edge tool to advance drug renal clearance and biliary excretion studies, which are important for drug-drug interactions and drug clearance. Specifically, the 96 multiwell insert plates, equipped with differentiated MATE1, OCT2, and OATP1B3-overexpressing HEK293 cells, provide a comprehensive solution for studying drug-transporter interactions. The HEK293 cells are pre-plated using a patented shipping medium and technology, which ensures full cell functionality and worldwide delivery at room temperature.

PreadyTake MATE1: Studying Drug-transporter Interactions Involving MATE1

PreadyTake MATE1 is an advanced model designed with precision, featuring 96 multiwell insert plates housing differentiated MATE1-overexpressing HEK293 cells. This cutting-edge tool empowers researchers to study drug-transporter interactions involving MATE1, a pivotal uptake transporter localised apically in both the liver and kidney. Unveiling a spectrum of capabilities, researchers can explore the substrate specificity, inhibition potential, and induction effects on the MATE1 transporter, gaining crucial insights into its functionality.

Moreover, PreadyTake MATE1 facilitates the assessment of transporter-based drug-drug interactions, shedding light on the interplay between drugs. The tool also offers a unique perspective on competitive inhibition and unexpected drug elimination mechanisms mediated by MATE1, making it an indispensable asset for researchers seeking a thorough understanding of pharmaceutical interactions. PreadyTake MATE1 is an advanced research tool that can unlock a new dimension in drug-transporter interaction research.

PreadyTake OCT2: Investigating Drug-transporter Interactions in Renal Clearance

PreadyTake OCT2 is a specialised model comprising 96 multiwell insert plates housing differentiated OCT2-overexpressing HEK293 cells. This innovative research tool is tailored specifically for investigating drug dynamics involving OCT2, a pivotal uptake transporter crucial in renal clearance. Boasting a range of key capabilities, researchers can explore the OCT2 transporter’s proficiency in carrying a diverse array of solutes across biological membranes, particularly pertinent for drugs subject to renal excretion.

PreadyTake OCT2 enables the thorough examination of substrate specificity, inhibition potential, and induction effects on the OCT2 transporter. PreadyTake OCT2 is not only ideal for studying compound interactions when drugs are co-administered but also offers an understanding of competitive inhibition and unexpected drug elimination mechanisms mediated by OCT2. PreadyTake OCT2 is a truly groundbreaking tool designed to illuminate the complexities of drug-transporter interactions in the context of renal clearance.

PreadyTake OATP1B3: Researching the Dynamics of OATP1B3 Uptake Transporter

PreadyTake OATP1B3 is a specialised model designed for the meticulous exploration of drug-transporter interactions. Comprising 96 multiwell insert plates with differentiated HEK293 cells expressing OATP1B3 and the parental cell line, this model offers researchers and scientists a lens into the dynamics of OATP1B3, a crucial uptake transporter exclusively located on the basolateral side of centrilobular hepatocytes.

Key capabilities of PreadyTake OATP1B3 include an in-depth examination of the transporter’s role in drug uptake into the liver, considering its significant expression on the sinusoidal membrane of hepatocytes. Researchers can explore substrate specificity, inhibition potential, and induction effects on the OATP1B3 transporter, providing valuable insights into its functional characteristics.

Moreover, PreadyTake OATP1B3 facilitates the assessment of compound interactions when drugs are co-administered, contributing to an understanding of competitive inhibition and unexpected drug elimination mechanisms mediated by OATP1B3.

Tailored PreadyTake Options: 1:1 WT/MATE1 and 1:1 WT/OCT2

All PreadyTake products are available in standardized 96-well formats using Corning plates. Tailored options, like 1:1 WT/MATE1 and 1:1 WT/OCT2, cater to specific research requirements. Timely delivery is ensured, with products shipped within two to three weeks after order placement. Researchers can also engage in consultations with ReadyCell experts to strategically plan and schedule their assays. El

Cutting-Edge Tools: Pre-assembled Cell-based Assays for Drug Transporter Studies in Preclinical Testing

ReadyCell’s Uptake Transporter Kits are designed to advance and facilitate high precision in drug-transporter interaction studies, featuring 96 multiwell insert plates with differentiated MATE1, OCT2, and OATP1B3-overexpressing HEK293 cells in line with EMA and FDA regulatory recommendations. With the patented gel-like medium technology, these pre-plated cell-based models can be shipped worldwide at room temperature, maintaining full cell functionality post-transportation.

ReadyCell’s unwavering commitment to high quality-control standards is underscored by available ISO 9001 validation data. The kits are ready-to-go with a straightforward four-step process: Receive the ready-to-use cell barrier, liquefy the solid shipping medium, apply the test compound through incubation, and conclude with the assay for permeability and transport endpoint assessment.

Features & Benefits of the HEK293 Cell-based Plates for Drug-Transporter Assays

• Ready-to-use
• Pre-plated with HEK293 Cells
• MATE1, OCT2, and OATP1B3 uptake transporter
• 96-well transwell formats
• Customisation options, such as 1:1 WT/MATE1 and 1:1 WT/OCT2
• High reproducibility
• User-friendly and easy-handling system
• Adaptable to automation
• Available on demand
• Transported in a patented shipping medium that ensures cell viability at room temperature
• Worldwide delivery

For more information please contact ReadyCell today: